LOCUS HUMAFP 22166 bp ds-DNA PRI 30-OCT-1994 DEFINITION Human alpha-fetoprotein gene, complete cds. ACCESSION M16110 KEYWORDS Alu repeat; KpnI repetitive sequence; alpha-fetoprotein; repeat region. SOURCE Human DNA, library of T.Maniatis, clones lambda-HAFP[26,33]. ORGANISM Homo sapiens Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 22166) AUTHORS Gibbs,P.E., Zielinski,R., Boyd,C. and Dugaiczyk,A. TITLE Structure, polymorphism, and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene JOURNAL Biochemistry 26 (5), 1332-1343 (1987) MEDLINE 87185438 COMMENT Draft entry and computer-readable sequence for [1] kindly provided by A.Dugaiczyk, 17-JUN-1987. NCBI gi: 178235 FEATURES Location/Qualifiers source 1..22166 /organism="Homo sapiens" repeat_region 339..435 /note="repetitive element X-1" repeat_region 564..664 /note="repetitive element X-2" prim_transcript 871..20359 /note="AFP mRNA" CDS join(915..999,1812..1863,2826..2958,5246..5457,6944..7076, 7995..8092,9641..9770,12046..12260,13918..14050, 14619..14716,15199..15337,16985..17208,18349..18481, 19820..19864) /note="alpha-fetoprotein precursor; NCBI gi: 178236" /codon_start=1 /translation="MKWVESIFLIFLLNFTESRTLHRNEYGIASILDSYQCTAEISLA DLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEK EILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMN KFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSL LNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLD CLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFL GDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLE CQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAI TRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANR RPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEE QLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV" sig_peptide 915..971 /note="alpha-fetoprotein signal peptide" /codon_start=1 exon <915..999 /gene="AFP" /note="alpha-fetoprotein precursor; G00-119-660" /number=1 mat_peptide 972..999 /note="alpha-fetoprotein" /codon_start=1 intron 1000..1811 /note="AFP cds intron A" mat_peptide 1812..1863 /note="alpha-fetoprotein" /codon_start=1 exon 1812..1863 /number=2 intron 1864..2825 /note="AFP cds intron B" exon 2826..2958 /number=3 mat_peptide 2826..2958 /note="alpha-fetoprotein" /codon_start=1 intron 2959..5245 /note="AFP cds intron C" repeat_region complement(4686..4911) /note="Kpn repeat" exon 5246..5457 /number=4 mat_peptide 5246..5457 /note="alpha-fetoprotein" /codon_start=1 intron 5458..6943 /note="AFP cds intron D" repeat_region 5918..5928 /note="5' Alu flanking repeat" repeat_region 5929..6232 /note="Alu repeat copy A" variation 6132..6133 /note="aa in [1]; aaa in allele" repeat_region 6233..6243 /note="3' Alu flanking repeat" variation 6421..6423 /note="ttc in [1]; tc in allele" variation 6452..6454 /note="aat in [1]; at in allele" variation 6652 /note="a in [1]; g in allele" mat_peptide 6944..7076 /note="alpha-fetoprotein" /codon_start=1 exon 6944..7076 /number=5 intron 7077..7994 /note="AFP cds intron E" variation 7421..7425 /note="agaac in [1]; ac in allele" mat_peptide 7995..8092 /note="alpha-fetoprotein" /codon_start=1 exon 7995..8092 /number=6 intron 8093..9640 /note="AFP cds intron F" variation 8403 /note="c in [1]; g in allele" mat_peptide 9641..9770 /note="alpha-fetoprotein" /codon_start=1 exon 9641..9770 /number=7 intron 9771..12045 /note="AFP cds intron G" variation 10364 /note="c in [1]; g in allele" repeat_region 11548..11850 /note="Xba repeat copy A" mat_peptide 12046..12260 /note="alpha-fetoprotein" /codon_start=1 exon 12046..12260 /number=8 intron 12261..13917 /note="AFP cds intron H" repeat_region 12822..13124 /note="Xba repeat copy B" mat_peptide 13918..14050 /note="alpha-fetoprotein" /codon_start=1 exon 13918..14050 /number=9 intron 14051..14618 /note="AFP cds intron I" mat_peptide 14619..14716 /note="alpha-fetoprotein" /codon_start=1 exon 14619..14716 /number=10 intron 14717..15198 /note="AFP cds intron J" mat_peptide 15199..15337 /note="alpha-fetoprotein" /codon_start=1 exon 15199..15337 /number=11 intron 15338..16984 /note="AFP cds intron K" mat_peptide 16985..17208 /note="alpha-fetoprotein" /codon_start=1 exon 16985..17208 /number=12 intron 17209..18348 /note="AFP cds intron L" mat_peptide 18349..18481 /note="alpha-fetoprotein" /codon_start=1 exon 18349..18481 /number=13 intron 18482..19819 /note="AFP cds intron M" exon 19820..>19864 /note="alpha-fetoprotein precursor" /number=14 mat_peptide 19820..19864 /note="alpha-fetoprotein" /codon_start=1 intron 19875..20214 /note="AFP mRNA intron N" repeat_region complement(21491..21501) /note="3' Alu flanking repeat" repeat_region complement(21502..21631) /note="Alu repeat copy B" repeat_region complement(21632..21642) /note="5' Alu flanking repeat" repeat_region complement(21955..>22166) /note="Alu repeat copy C" BASE COUNT 7164 a 3625 c 4062 g 7315 t ORIGIN EcoRI site; chromosome 4q11-q13. 1 aattcaaaaa gttcccccag tgattctaat gtgtagccaa gatcgggaac ccttgtagac 61 agggatgata ggaggtgagc cactcttagc atccatcatt tagtattaac atcatcatct 121 tgagttgcta agtgaatgat gcacctgacc cactttataa agacacatgt gcaaataaaa 181 ttattatagg acttggttta ttagggcttg tgctctaagt tttctatgtt aagccataca 241 tcgcatacta aatactttaa aatgtacctt attgacatac atattaagtg aaaagtgttt 301 ctgagctaaa caatgacagc ataattatca agcaatgata atttgaaatg aatttattat 361 tctgcaactt agggacaagt catctctctg aattttttgt actttgagag tatttgttat 421 atttgcaaga tgaagagtct gaattggtca gacaatgtct tgtgtgcctg gcatatgata 481 ggcatttaat agttttaaag aattaatgta tttagatgaa ttgcatacca aatctgctgt 541 cttttcttta tggcttcatt aacttaattt gagagaaatt aattattctg caacttaggg 601 acaagtcatg tctttgaata ttctgtagtt tgaggagaat atttgttata tttgcaaaat 661 aaaataagtt tgcaagtttt ttttttctgc cccaaagagc tctgtgtcct tgaacataaa 721 atacaaataa ccgctatgct gttaattatt ggcaaatgtc ccattttcaa cctaaggaaa 781 taccataaag taacagatat accaacaaaa ggttactagt taacaggcat tgcctgaaaa 841 gagtataaaa gaatttcagc atgattttcc atattgtgct tccaccactg ccaataacaa 901 aataactagc aaccatgaag tgggtggaat caattttttt aattttccta ctaaatttta 961 ctgaatccag aacactgcat agaaatgaat atggaatagg tgagatattt tgtgtttttc 1021 ttgtcttttc tctatatcaa aattttttaa attataaaat ttgcattaat ttgtcttgat 1081 ttattattca tatttattat tccacatgga gaaaaaatat ttaactgatg gatatattta 1141 aatgaaagaa aaacttgtaa ctttacaaga ggtttacaaa gttatagcag tgtttaatgg 1201 atgaatggtt tgtatgtttc atgttgaatt aatttttaca cttcaatggt atgcatatta 1261 actttgaaaa attatatata tacacatata tgtacatata tatgaatata aataaaattt 1321 tatatgtgaa gaagccagaa ttatgctcct tcacataact ccctcagact agtaaaatag 1381 ataaaatctt tgtttttaat acagaaaaat gggtcattat ttgatggtct gaagaagaaa 1441 tattgtgact gggatatgaa tggcaaaccg tagtacaact atgttcaaaa gaatgcctga 1501 aatatatttt taaccatttg actttcagga cagttacagc actacagtac agggaaaaac 1561 caaacaactg gaagacaaaa tctggatttt agtgataggt ctactataaa ttatgcttgt 1621 taacttcatt ccttagtttc ctagttttct tttcctcaag tataaaatta agatgcttag 1681 gttatcccta atgttctttt aattctgaaa ctgtacagtt ctaactgaaa cacaaacatt 1741 catatgtaac aatgattact ttcttggttg cagttgaaaa cacgtttcat gaagtttatt 1801 ttgccttcca gcttccatat tggattctta ccaatgtact gcagagataa gtttagctga 1861 cctgtaagtt ttgcttatat aaatgtactt taaatgtgta aagcaaggat aagtaaatac 1921 ttaaataaaa ttgggtaccc ctgtgagctc ttaaaagcac aaaagcaatt tggacaattt 1981 caagaaaagt tactcatact gaatatcaac ttgatgttga agaggttaaa ctgttgacta 2041 atgtcttcga cattgacctt ttgattcctt gaaatctcat gagtcaaacc aaatcagatt 2101 ttagaaactg aagattagtg tctgatcagt gacaaccata tactaattca ggaatttttc 2161 tcatcagtac caacagggtg atattataat gttttctttt ctgtatacta tttaaatctt 2221 agcagcaaac cataggtgat aaaatattct atttgctgtt atttgtggag agtatgttag 2281 tctcttggat gtctttccat tccacatttt aaaaatttct aacaaagaat ttaaagtagt 2341 gtgttgctgt tactccttgc acatccaaac ctgcataagg attgctttga gtcaatccat 2401 gagcactgta gtcttgggtt ttagaccttg atcatactgg gaatagacac tgttagaggt 2461 ctgtctaatt accaattttt ttttgcttaa atttaaaagt aaccataaag aatatagata 2521 ccctcaatta tgggtacatt acagtagatg gatggtcaca gaaggagaaa ccactcttat 2581 gggaaatcca cttattttag cctttaacat ctatatgtat atttatggca aaagaaaaca 2641 agaaaaagac taaagtttct tctcagatga cctggaagct aattttacat aattttacaa 2701 atcaaatgtc taaacagatt acaacataaa tagaaaacaa aacaaacaaa tgaaaaacta 2761 tacttgagaa aaataagctt gctgcaggtc tgttccttaa ggattcacac gtatttttgt 2821 ttcagggcta ccatattttt tgcccagttt gttcaagaag ccacttacaa ggaagtaagc 2881 aaaatggtga aagatgcatt gactgcaatt gagaaaccca ctggagatga acagtcttca 2941 gggtgtttag aaaaccaggt gagtgaataa ttttaaaaaa gcattgtgat atttgacaaa 3001 aatttagcat gctgaagaga agatacaaaa atagcagtga aaaatgcatt taaatatttg 3061 aagagctatt gtatgaaaga gggattagat tcattctgaa ttgctaaaga gggcagaaga 3121 gaacaatagg tagttattat aaagagacca tataaatatg atgaactaag gttctgaaat 3181 aagattatct tgatgactat gggcatatta acttttttga gcttcagttt tcttatctgt 3241 aaaataaggg atgataatag ctcccatttc atagttagca tggaaattga tataacagca 3301 atagtagcta acttttatta tacacacaat gtgactggca ttattctagg gagcataatg 3361 tgtatattga taataaaaat attttatgac ataggggata gatagcactg atgaatcaga 3421 atggttgtcc agtgagtcaa gagatgctgg ctcgggcttc tgggcaggat atcagctttg 3481 cttacctata tttatttatt aaacatttaa aataatcctt gaagatagat gctaatcttc 3541 caactgagga agctgaggct cagagaattt aagtaacttt cttatgggaa ccaccaaatg 3601 gcagagccag gatttgaact agaccatctg gcttaaaatt gacagtctta gtagcttcat 3661 tacactataa ctatagtgaa tgtaagatgc atagcacatc gttagggttg ccaggtttag 3721 caaacaacaa caaaacataa tacccagttc aatctgaatt ttagataaac actaaatact 3781 tttcttagta taaggatatt tcattgtaga agctcaacaa ataatattta ttatttattt 3841 tatctcaaca tagaaacaaa cttgataatg attagaactc tccaattata aaacaacatg 3901 cccagagaat actctgttat ggtggggtta attaggtggc tgaaagacaa tgtacctgga 3961 atatcataga agagatgctc ctttaaggat atagtttaag ttctttccaa ctttgaaatt 4021 tatgaattga caaaaatttc tgttttgcat ctctattttt gtcttgttct gataatcttt 4081 tcaaaatgtg tataaaaaaa caagaataca ttatctattg caactttaca accaattaga 4141 ggttcaaggt aatgttacag atcgctgatt tattcttgta aattcaaagg tatgtctttt 4201 aaatgaggat tgggaattag aaatcttacg taagccttcc aggattctct aaatattact 4261 gtagcagcta taaaagctac ataaaagttc cctcagatac atgaaacaca tgtattcctc 4321 agatgctttc tgtggaatat tgatgctgtc atctgagttt ggtaagggta agtcacagag 4381 gaggaaacac atacatttta aaacatttta gctaaatatg taattgtggc caagaaaagt 4441 gtttttttaa aaaataatta tttcatttca aaatcatttt tatttataat tgaaaataat 4501 atgcagtttt ttattgtctt gtaaggatgg catgtaaaat gagcatttat gtctgaaatg 4561 tggtatgtct gtgtgtgtgt gtgtatatgt atatatgtat gtatatacca taatatatac 4621 atatgtattt gcaattccaa aagttacatc tttaatgaga atcatgaaaa tattattttg 4681 ctcagtttct ttttttattt aaatttaaat ttaaagttct ggtgtacacg ggcaggatga 4741 gcaggtctgt tacataggta aacacgtggc atggtggttt gctgcaccta ccaacctgtt 4801 gcctgggtat taagcccagc gtgcattacc tatttttcct aatgctctcc ctccctgcac 4861 tccactccct gacaggcccc agtgtgtgct gtttccctcc ctgtgtccat gaaacatggt 4921 ttatatattt ataaaagttt atatatccta gtccaaattt ttgataacac aaaaaggaaa 4981 aataaaataa ttcaaaattg ggaaagagaa acaaaagatt gcatggcttt tttcctttta 5041 ttgtttggac attaaagtct cattttccat aaggcagcaa agaaatctat ttcatcaggc 5101 tgaaacaaaa tacattagaa tttgtatgga aaatttttca gaatctatag ttctgatttt 5161 agacattaga aaatgtttca tgtgtcttat agatttttaa agcaaagtta gttgtctttc 5221 tccaaacata aaatatttct tatagctacc tgcctttctg gaagaacttt gccatgagaa 5281 agaaattttg gagaagtacg gacattcaga ctgctgcagc caaagtgaag agggaagaca 5341 taactgtttt cttgcacaca aaaagcccac tccagcatcg atcccacttt tccaagttcc 5401 agaacctgtc acaagctgtg aagcatatga agaagacagg gagacattca tgaacaagta 5461 aggatccagt ttaaaggtag atgcaaacct cagaaacaca gcaatggcaa gcctaattta 5521 gtatttttgc aatgtactca tgtactccca gtaagaggta taatgtttct ttggtgttgt 5581 gtctgctgag gtcccaggca aggtaattag gagagcaaca ttcaatgtaa cttggtttcc 5641 atagcacgct agatgtagta caaaccacag gacaaaccta ccagaggttc cattagtccc 5701 ttgagagata tacacctttt ttttttcttt ttacccattc tactctcaat tttaccttgt 5761 tcaagaatat attagtattg caccaaattg atgctttcca gagccatata ttggtgtttt 5821 gtgttcacct aattgcctag aaataaaagt tagagatcca agcatggtca agcatggtca 5881 agatacacag acatggcaaa ggtttactga acacttagga tgttgtgggg ccgggcgcgg 5941 tggctcacgc ttgtaatccc agcactttgg gaggccgagg cgggcggatc acgaggtcag 6001 gagatcgaga ccatcctggc taacatggtg aaaccccgtc tctactaaaa atacaaaaaa 6061 aattagccgg gcgtgatggt gggcgcctgt agtcccagct actcgggagg ctgaggcagg 6121 agaatggcgt gaaccctgga ggcggagctt gcagtgagcc gagattgcgc cactgcactc 6181 ccgcctgggc cacagagcga gactccgtct caaaaaaaaa aaaaaaaaaa aaggatgttg 6241 tggaaacatg tctgcttgca cagaggatca gattaacact cacaaacaaa ctttgaagcc 6301 tccttctcct cttcctcttc attcttcttc tttcccccat tttgttgata ggaaattgaa 6361 ggttggaagg ctaaaacaac tggctaaggg cacacagcta gtacatttga ccccaggttc 6421 ttctgttgga gaagcctgta cgtaactctt caatcacttc tgccttccat gttacttcct 6481 aaaccagata aatagagaga ttgcccttag aacatctctg ctatggcgac tatccaggtg 6541 cataacccca ttctgcactg acaagggata aaatgtccat ctttctgttg cacttagcag 6601 aagtctggct ttgctgatcc ctgaaacata tctgagtagg cttattgaaa aagacctttt 6661 aataagaatc atggttagca tgtctgccta ttttcttctc ataatagaat ctggtaccat 6721 ctgtcagata tttttccccc caggatcttt agtgaggaaa attttgacaa catgtggaaa 6781 aaatatgatg attttcccct taggaacaca gtaaagacaa agtcaaatgc attttgttgt 6841 tgttgttttt gaatctttaa ataaatccca gtgtccagtt ccaagcagta gtagtcctat 6901 ttttaggtgt ttataaatct tctagctcta ttttatttca cagattcatt tatgagatag 6961 caagaaggca tcccttcctg tatgcaccta caattcttct ttgggctgct cgctatgaca 7021 aaataattcc atcttgctgc aaagctgaaa atgcagttga atgcttccaa acaaaggtat 7081 catatttgcg tggatatctg aaccagtact gtagtctatg actcattaaa acaaaacaaa 7141 gttaaaaatg aaaacgtgct taattgtgga gagtatcgtt tttggaatag agaaatagtt 7201 cagcagtctg atattcttcg agtgaacaaa actggatttg ctggttttta ttatctattc 7261 attaagtcaa caataatttt attattaagg aagtgagttt gatgggataa agaaggaaag 7321 acagacagac agaaagagag aaagagaggg tgctataaat gggaaggagt aagtgaaaga 7381 aaacggagaa aaggaggagc agaggagaga gaaaaaatga agaactatga taaatgcttt 7441 atacttactg tttttttaaa ccttagaaca aatttgaggt gtaagtattt ttgtccttta 7501 acaaactagg aaagaggttt agagaagaaa atttttttta ggtttagaga ggtagaataa 7561 tatccaccat taactacttg ctaccagcag tcctataaca aaaatttcct gagaactgac 7621 tactctcagt gtcaagccgt gatacaatcg tattttttgt aactgagaag acacttaaga 7681 aattagcagg cttcaatttg tctttaagct gtttaaaggt acagttgttc atttatgatc 7741 cccagtataa aagttatgtt tttgtttcaa ggtactttga gtaaatttgt ctggcacaga 7801 tgcatataaa ctaacccaaa agaataaatg agtcaataat attctgcgat aatgtatgac 7861 atttataatt tttagtaaaa aacatatttt atggaatttc attttaccta tacttgttgt 7921 ttttctaaat attagagctt gtaaagaaaa tgttagtata tgctttcatg acattttgtt 7981 tcctctacat ctaggcagca acagttacaa aagaattaag agaaagcagc ttgttaaatc 8041 aacatgcatg tgcagtaatg aaaaattttg ggacccgaac tttccaagcc atgtaagttc 8101 aagttctatc tagggaagag ggtgagagct acagaactac cattttgcaa tttgggttcg 8161 tttttttaat tgttgctgtt ttagagaatg aagacccctt tgtgacccct ttgatgaggg 8221 ctaatgggat tagaaccatg aactcttagg atcagaagga agctaacgga taagtcagtt 8281 taacacttac taaagcctag ctgagataat acatagaaaa gacttttaaa gtttatgtta 8341 tttacctgtt ctttaagaca cttaagttct ggcctgccat caaattatac ctcatcacta 8401 gaccatattt ttctagctct tctacaaaat aagtcagcct tcactgagtg tcatttaaaa 8461 cttttgcctt aacaagaaat tctttatatt aattgtgttt cttaatcttc tataaggctc 8521 tttatagcat ttattgcttc ccataaaaat attcctttga ggcaataata ttagaatcta 8581 gtgtcaggag aaaagaacat tttaaattat ataacttctt taaagtactc atcaactctt 8641 ttatgataaa acatttctag tatatgaaaa tatctgagct gctaatcgaa tagtagtaag 8701 tatatatatt cagagtttat ttgattgctg tttggttaaa taacacgtta aagcatattg 8761 tagacaatgg aaatctagaa tgaagttttt agtaatagaa ttagttctaa agactgaaat 8821 ttcttcttgt aagaagtcag atttatgcct taactaccct ccaactcaat tagaaattga 8881 aagattaatt tatgacctac tttaaaaaaa tttatcttag taataaaata gaaattgaga 8941 tagtattata atacccttat catttgctat ccataagtga aagctaagtg gtctaaattt 9001 atagagaaga tccatcttta tcagaagcag gagtaacact attttctcta ggagcaatgg 9061 ttttacaaag acaactctta aaatttaatg aaatatgcag aggaaaccag aatttttatt 9121 tcttacttct tttttggcca cattactctc ttggcacact ggtatgcctg aaatgtttaa 9181 tctgctccac tttcttgtcc catttcattg atttgcacat acgcctcctt tcctggattc 9241 tcagaagtat tttttacccc agtaaaaaat ggttctcatt ttcatggaat tttccatttc 9301 ttaattgttt atccttttaa agttttatgt ccactcaata caaatgctca gcataagggt 9361 tgaatggcaa aaataattta ccttaattct taagacatgt tttaggaaaa gataaattat 9421 ttctaaacca tttgtggggc cagctcacag tttacagagt taccaccttg aaagatatgg 9481 ctggaatcaa gccttaaaac atgtttcctt ttctttttaa acaacaaggg aatttcagtc 9541 atttctcttt gaaacttcta ctgtagtaat actctataaa ttctatttta ttttgacaga 9601 taaccaagaa attaatttct aatttctttt ttttccctag aactgttact aaactgagtc 9661 agaagtttac caaagttaat tttactgaaa tccagaaact agtcctggat gtggcccatg 9721 tacatgagca ctgttgcaga ggagatgtgc tggattgtct gcaggatggg gtgaggagtc 9781 ttgcttctta aaatagaaga ttttcactcc cttttctttc tttttgtctc attctaaaag 9841 ggagaaggtt gtttgacttg aattggttac agagtatgta aactaggtga ctccttaaat 9901 ttgcagaatt ctcggtagta aaacttaaac catcttttgt tgatcctggc tttcacttta 9961 gctatacccc tttttgtgaa accaaggctc atctatttct tacttctaaa aaaaccgtgg 10021 gaacttctca gaaggcttct ccatagttac ttggaggacg ggaggaaact aaggtttaat 10081 gtatttattt tttcattcat ttattctttc atttgacaaa taaatatata ttaaatactt 10141 tctatctgct agccactatg acagacactt gttttaaaag cacaggctga cctcaaggaa 10201 ttcacagtct gataggagag ataagacagt gacctccttg gagttaggga ctgccttggt 10261 ttactgttat ctccatagca caatgcctgg cacatggaag gcattctata atagtttgtt 10321 aaatgaacga atgcaataaa aattgcacaa gtaactgtcc taccaggtaa aaagctagcc 10381 ttgccaaaga caagtgtgaa taaagtggtc tcggagaatt agaaaacaaa atttaaaaaa 10441 cccagcaaca gtttcttgag tgtgctctag tcagtggtgg tttaagcagt gtggcattgg 10501 cttattttgg ctaaccctag acttcctaga ttttacaaga gaggactgtt gaccctcaga 10561 ttactctgtc tctgtgggct ccatgacaca ccaaagagat taaaatccaa ggagcttaaa 10621 aattactgct cttaggtact caaatggctt gataaccagc actggactat ggtttccaga 10681 aggctgaaag tgaagataaa atgctcattt cagcccatcc gatggcaatt cagtgagatg 10741 cttgaagtaa gcaagaagcc gaggctgcag aggaggcctg agagtgacag tccctgagga 10801 gctggggaag aagtgggagg aggcagcctg gcaggcgact gtactacttt acttgttttt 10861 cttctagaaa tatggcatac taggaaaacc aacaagaagt attttgtttt tctttgagct 10921 cagttttccc attttgaacg gacaatttta ctgtttctcg ttgtcatttt taaaaagtta 10981 attttttcaa tttttggagc tcataaccac ccttttcctt taaagtggaa acattaattt 11041 cagcatgata tgtaagttgg attttgatag ctgaataatg ggttctaatt atctttgctg 11101 agaatgtaca gaattttcag tcccatgaca ggtatatatg taagctctgc ctctctctgg 11161 ccacttaggt gcattgccat tttattatct atagactgcc ctctgaaggt catagtcagt 11221 cactgcagta tgttctgata aagatgatta tcattcttac ggaatttctc gtggagcaga 11281 aagtttgctc tccatgttat gataccagtt gcaagtgttg tttaggggca aatttgaatg 11341 ctaatagaaa tacatatagc aacatgcatc ctattttatt tgagcacatt tccctcttat 11401 ttgtaaaggt tttcaattga aataacatag gatttagctt acaactatgg aaagaagaat 11461 tgaacaaaca ggtaagtgga aaggaatgag aaaaggcaaa agtggggaga aagcactaaa 11521 acgggagaca agttaaaatt tctttttaat tgataggtca cgttctcact ctatttgcct 11581 ttaagggaag aaagcaatca agttaatatg ttttccttca ttgtatagta tgtaactacg 11641 gacactatta gaggagggat ttgtgtagca cttaggacat tatacttgat aatttccaag 11701 ggtctttcta gatttaaaag tctgattcta acgtagtaat aaaaataaag gcccaatttt 11761 ctctttaata ttgcctgaag atattactct attattgcat taaaattaaa cattcacaca 11821 ttgtttgcac tgctaaataa aattatgtaa tttcttcttc tttccttcct ccttccccca 11881 tccctctcta tttccctttc cccttccttc tttcctggcc ttttttcctt ctttctttgt 11941 tcccttctcc ctccctcccc tttcttcctt tttctaaagc tggctttgag atcctttatt 12001 aaagaataaa tctttaaaac ttatacttta ttttccctgt tgcaggaaaa aatcatgtcc 12061 tacatatgtt ctcaacaaga cactctgtca aacaaaataa cagaatgctg caaactgacc 12121 acgctggaac gtggtcaatg tataattcat gcagaaaatg atgaaaaacc tgaaggtcta 12181 tctccaaatc taaacaggtt tttaggagat agagatttta accaattttc ttcaggggaa 12241 aaaaatatct tcttggcaag gtaacacact ctgtaaatgc atgttcatgc aagtaaaaat 12301 gattatgtgg ctgacagatt tgcgttgttg aaatggagag tgatgattat ggtttttgag 12361 ttcaatatgt gaggatattt ggctagaatg ttctgagcca aaatagattt cagtagataa 12421 ccagggaata agtaatggga tttggtgttt aacggtgaag cgttcaccac tgtgactcat 12481 taactgcttt gctatgaagc tgaattttat ttcacatcaa tttctctgga atcagaagca 12541 ttgtcatcct gtaaagatta ctcatatcaa ggccaccatt gaactctcaa ataggatatg 12601 gatatttttg taataagaag agttcatgat taagaatgaa ctcttgctac gcatgttaaa 12661 aaaaaaactt ttctccaaaa gataacacaa gagataatgc taggtagaag aacttttata 12721 ggaacagctt attggctatg tattaaatac atgttttgta ttttttaaga aaatcaaaac 12781 atgtttagag acatttgcag tacagtagtt tgttttaata caactgatag gtcacgttct 12841 cactctattt gcctttaagg gaagaaagca atcaagttaa tacgttttcc ttcattgtat 12901 agtatgtaac tatggacact attagaggag ggatttgtgt agcacttagg acattatact 12961 tgataatttc caagggtctt tctagattta aaagtctgat tctaacgtag taataaaaat 13021 aaaggcccaa ttttctcttt aatattgcct gaagatatta ctctattatt gcattaaaat 13081 taaacattca cacattgttt gcactgctaa ataaaattat gtaagctaga ataaagttca 13141 gatttaggag acacatagtg acaactgatt ggtgacagaa ctaatcctat aatctgggaa 13201 tacggttagt aaagtcaaga attaccttta agtttacaca tccatgcaca tctaaatcta 13261 attgtttaat agaagcagtt cttcagttgc aaaggttctt tgcagtagaa ttttctcagc 13321 caggaatgat tttcccccag atatttgcat ggcttctttc acttagctga tctctgttct 13381 gatatcagct gcctagagag aattttcttg accacattca aagttagtgg cctctccacc 13441 ttgggtatca tccttttttc tctttttcat ctttatttat tttcattgat ttatcgctaa 13501 ctgaaatgag atggcctatt tcttgtttat ttgttctgcc tccctataat gtgtgctttt 13561 cagagggcag gtatttatct tagacatcat tgagtccgtt ctgcttaaag caatgctagc 13621 aaagagtgga cactggaaaa atatttgttg aataaatgaa tataaagtcc gtaattgaaa 13681 agtcaaattg agagatgcag gagaaaacaa aaagccattt tacaggacaa tttgaaggat 13741 cacagtctgt attaacagtt ttgccattca tataattcaa atcatatttg attttcaggt 13801 ttatttattt gaatttaact tccacatgcc atattatata ggaataactg gagaagtgat 13861 ggctcctttt gtctcttagt tccaataact tgaaatattt ttctccacat atttcagttt 13921 tgttcatgaa tattcaagaa gacatcctca gcttgctgtc tcagtaattc taagagttgc 13981 taaaggatac caggagttat tggagaagtg tttccagact gaaaaccctc ttgaatgcca 14041 agataaagga gtaagttgct ctagaatttt aggggagtat gaaaaactgg attgatatca 14101 tctgttaaaa atgctgtttg tttgaaagcc tctagttttc aactagttgt tagccagtta 14161 tatctatttg tctagatatt aagctgttat taactagcag tcagcagcta gtggcttgct 14221 ttagaaacaa aaatgttaat tgcttctcag ccttttggct aagatcaagt gtagaaataa 14281 aaatgttaac caaaagtcct ttgatccaca aataaaggta gtattcatta ttcatttttg 14341 gataacttca gaaaggcaag aatttggtac agaaagaact gtaaccattt atccaaagat 14401 tgagttttgc cattaaatga ttttgtgatt tataaaatgt taaacttaat ctccccaaaa 14461 tccattttct gtaattatca aaatttacac tttaccatat ttaatattta aacatctctg 14521 attggtttta taatagtata taatattgat caattttata tacaaagtta tgcatccaag 14581 aaaagaaaaa tgtatatgta ataattcttc attttcagga agaagaatta cagaaataca 14641 tccaggagag ccaagcattg gcaaagcgaa gctgcggcct cttccagaaa ctaggagaat 14701 attacttaca aaatgcgtat gtttttgtaa acagtatttt tagtgaatta aaattattaa 14761 agagaatgta gccttcccca attctcctcc tttggaagca acaagaatga cctgtgaggt 14821 ctgatctgtg gtattgactt taagttcccc atactgtgca aatttttgca gtaaagatat 14881 ccattctgtc atagtctgtc cgagttaaag caccaaaaga tcacagttaa aatcaatgaa 14941 gctccgaggt tgagaataca agtcaggctt cctcctggag tttgcttttt cattgtgata 15001 tgcttctata ataggagtac agggagtggt tgttagagta aatgcatctc aaaagttggt 15061 tcaaatacca tgtagatgaa acaaacgagc tgacctcatg tcttctggca tgagagtaga 15121 gagtctgtga gaagaagcaa ggcggctaaa aactcatgaa tgactcagca ggacttagtt 15181 aaaaaatgct tctttcaggt ttctcgttgc ttacacaaag aaagcccccc agctgacctc 15241 gtcggagctg atggccatca ccagaaaaat ggcagccaca gcagccactt gttgccaact 15301 cagtgaggac aaactattgg cctgtggcga gggagcggtg agtgtctgct tggtttggtc 15361 ccatctcatt tctgccctgt ttgacttgaa atagcctcat aattcccctc tagggaagac 15421 ggtaaaaacc aatgtagaga tggccttagg aggcttgttt gattagtcac ggttggaggg 15481 gtgtgagaac ccagctctgg atggctggca tgtggccatg cttcctattc ctccagggtg 15541 gctggtggaa gttcagccag tttagtcaac aatatctgag ccaactttat atatcagaaa 15601 gacagaacga ccaacatgta actcataatt cataacaatt cataattcat aacttcaaat 15661 cataatttct gctttttgtt catatactta ctttgatgtt ttaaaaaagc tttatctttg 15721 attgattaaa attagtcatg ctattttagc catatttata ttttcacctt ttgtaacatg 15781 atattattat tatgacatca ggaataattg gttccctttc gcagggtata gggtacagca 15841 caggataagt attatgttct gaatagtaaa atgactttcg agtcagtaat gccaatattc 15901 tttacttcct aatgtcacta gtatcataca taagattaca ggatgaatta aaaatatttt 15961 tcctataaag tcataattgc aaacaaaatt gtctatttta tcctttttcc tctttttcat 16021 aatggggagt tatttgctgt tagtcttcat gtcatacatt ttttccccaa aggttaagag 16081 taaaaggaga gttcttgtga ttaaatgtca cctcaattgt ttgttgaatt tcccatgctg 16141 ggaggctgca gggatgcagg atggtgtaat ggttcaggag tgtatgttcc ggaggccacc 16201 agccaacaac cgcattctac tttcactatt ctttagttgt atcacagtgg gaaagcaact 16261 tatgtcatta agcttgagtt ttttcatctg acatttgaga atacaaatta taccaccctc 16321 atacgacagc tgttttaaac aagataatct gcataactca cacagcacta gtctgacaga 16381 taaagtgcac acaaaacata ttatttctta ttacaagtta ttactaggtg attaagaaat 16441 atctcctaag taggcaaggt agcaagattc tacattagga aagtcttaaa aacccacaaa 16501 attgctctta cttcttttca attaggatga tatattagct gcaagtgtat acatgtgtat 16561 atgtatgtga ataaaagggg taagtttgtg ctattcttac cttcagatag tgattatcaa 16621 aagaaaaatg gaaagttcaa ctaaatacac atgggaaaca taaaggcaga gacatttttg 16681 tcctttagaa gtgtgtatgt aactggaagc atgtttcaaa tagctgacac aaatagctaa 16741 atgactatcc tcaacatcac atatggacca tctgctacta cttgctaagg cttagcccaa 16801 acaaatgggt aaatcctgga atttacaata taatgtcaca tgatcctaca tagcaaattt 16861 tcctgtaata ttaattataa attgctgggc attagaaatt attgcagcag ttttctgaaa 16921 aactgaacca actttgtgac taatgcccaa tctccttact tttttttctc attctcctaa 16981 ccaggctgac attattatcg gacacttatg tatcagacat gaaatgactc cagtaaaccc 17041 tggtgttggc cagtgctgca cttcttcata tgccaacagg aggccatgct tcagcagctt 17101 ggtggtggat gaaacatatg tccctcctgc attctctgat gacaagttca ttttccataa 17161 ggatctgtgc caagctcagg gtgtagcgct gcaaacgatg aagcaagagt aagaaactgt 17221 tacttgctag catggaaaag aatgacaacc ccaaagagta actgagactt ctacctcgct 17281 cacctaacac tattgggctc actaacagag cgttactccc aaaacactta aaatgccttt 17341 gaaaatagtt ttgtctcagt gtcttcacag tctcattggg gaagcaggtc tagaaaaatc 17401 gacgagggtg gacaatttcc tgtttgtaaa aataatctct gttgtaactg ttattgtgat 17461 atgtatttgg gggttgagga aaagtgggca atctattctg aggaattaga gtgtatcttt 17521 gcagcaaatt tgggtacttc cattccaagc acaggaaaca catcattgaa tcttttttta 17581 cactatttac actttgaaga gaataaccat cttatttaat tcaaccatgc agtttgggtg 17641 ttaagaaatg acatgtacat ttcagttcat tgtgggagct cttttgtaat ggtgatggtc 17701 atgcaagtca atggagctta tgttcttcaa actcccatgc attttaatcc tcacttgttt 17761 tgtaaatagt cttccttcat tggaaaaccc attcttctct tttttcctct atcacagtct 17821 gaggtatgtt tcacagtatg ataagaatgt tgcctgttct ggcaagcttt ttctattgct 17881 ctggtctact ttctattgct ctggtctaag tccaacatga aaggcttgct aagtgagcag 17941 tgcaggcaat tagtgctgcc agtgcccaga taaggggtgt gataactgga tgggcaggat 18001 tcggagatct gggtctttga gtgtagataa gacacagtta agaagagcgg acaggaaagg 18061 atattcctgg gggatgaggg gagattgcct tccactacac ataagtatgg tcaagtatga 18121 aatagtgttt tatccacaac ctgcacaact ccaggctggt ggaacacttg gcatgttttc 18181 agcctcaatc tttctactga aagtactaga caaggtgtgt gtggtcagtc tggtgatagg 18241 ttgatggagt aagggtttag gctctgaaaa ttctctacta ggaaggctgt agaaaaatag 18301 cattgcataa cagacttctc ttgtattttg ttttgtttta aatcacaggt ttctcattaa 18361 ccttgtgaag caaaagccac aaataacaga ggaacaactt gaggctgtca ttgcagattt 18421 ctcaggcctg ttggagaaat gctgccaagg ccaggaacag gaagtctgct ttgctgaaga 18481 ggtacatgca gctcatttca tactcaaaat acttgctatg gaattttctg tagtggataa 18541 tgaaaggaag accctacaaa tttataactt taaaatattt tcagagagat ttaaatttca 18601 ttgagaagca gattgaggga ttctataaga tttaaaaaat aatcacattt tcttgcttaa 18661 tattaggaaa atttataata ttaaaatata ttaatagaat tagtaatttt aatttatttc 18721 ctagtagaga aacccataaa gtgaatgtgt aaataattga tggtaattta gatagtttct 18781 ggcctaaaat tgatcaattc agctaaatgg attaaaggat ttaatagcaa attaattgtg 18841 caaacagagt attaggagtc tatttgtaga aaatgttttt gaactcattt agaagcttgc 18901 ttttgtacat caacagagta gtatttagga gttattttaa ttacatagta attttagctg 18961 gataattagc cagattttct ttaaccaggg gattctacct aacatttaaa aaaattacct 19021 tttttcagct ttattgaggc atgattgaca aatacaaatt atatatgttt agggtgaaca 19081 tgtgatgttt caatatattt atacattgtg aaatgattat cacaatcaag ataattggct 19141 aaattttaca aatctttagt ttgtattgct acatatattt gaatatagca acactatact 19201 ttaaaaagat attctataac ttagcgtttt tgtcaatttt acctttctca ccatgtaaaa 19261 tccaaagaca gatatattta gaaatgtaga gtttttctat aaataatata attagatgca 19321 tttgagtgtg tgcacttacc agtatatgtg tgtgtttttg gtgggatcag gtagggtggg 19381 acatagataa ccaaattaga taaaactggt gaaacagatt tgatgtgaag catttctgaa 19441 aaacatgaca caagaagatt aatgttctct aatctgagaa gacatttatt tagatataga 19501 gaacatgaac aaatagtagc agtgctttat ctgcaaacct tttaatttct aataacttgt 19561 aatttgtaga ggaaggggaa agattgagaa tacgcattga tttggagatt gttatagaag 19621 aaaactgttg atgtgaaaga atattgtttt ctccctggct tttactatcc caggttgttg 19681 gcatcagaga tgtgtttctt catttttaac ttagttaatc tacaaaccta tgaattcacc 19741 ccggattgta gagtgttaac tgtatgattg gtataataat ccatttcttt atctgattat 19801 gtttattctt aattttcagg gacaaaaact gatttcaaaa actcgtgctg ctttgggagt 19861 ttaaattact tcaggtaaca aaacattcag acaagcctga atacaatgtt gtttctccag 19921 aaatatcaat ccataatgag atagatcatg aggagtgcca ttaattctct taaaaataca 19981 tggaattcaa aaaaaagttt attttaaaaa cacttgaaca aaattacgca cacaatgtta 20041 aattagtggc tcaactatgc aaaatccttt ttggttattt aaaagacttc aacaaatgct 20101 atcagaagac tttcctacgt atccaatatt tctctgatat aaaataatag aaccagttac 20161 ttactgcacc tattagttta attagtattt aatatatttt tgctcatatt gcaggggaag 20221 agaagacaaa acgagtcttt cattcggtgt gaacttttct ctttaatttt aactgattta 20281 acactttttg tgaattaatg aaatgataaa gacttttatg tgagatttcc ttatcacaga 20341 aataaaatat ctccaaatgt ttccttttcc aagtttgctt atttatgaaa agttatcgat 20401 aatttcttta gttttgtata ccattgtctg aagcagattc tgttaaaata gcattaagtg 20461 ttggttgtta taggagatta aagctatcca aggatggatt tacagcacta gatcacttgg 20521 tgaactgaaa aatgttccca agttaaacac tatttgatgc taccagggca ttttgtttat 20581 taaatgacca tcactgaagt attctaacag ataatctgga gatgagaaaa gaaattatta 20641 ttcttctatg ggatctaaga aaatttaaca tctacttttt ttctcaatct tgttcagttc 20701 tctattcaca gacattttaa aacataagaa tattacagtt ttgattgaat ataattaatg 20761 ttttcatcaa ttaaattttt attccacaaa tgtttattaa gccttcactg agttccaggc 20821 cttgggctgg gtacagtcac tgttctccag tcgcttcagt agagaaggaa gcaacacaat 20881 aatgatgcct cagattatga ccacaaggag ttgacaacaa attgttgagg gaacgaaagg 20941 gtgggtgaca tcagttctga ctggaaaagg tgccataaat gtttaagaag agtcttagaa 21001 aatgaatgag cattcatcag ttcaagtaaa agagaaggcc attttatgaa agaggaaaca 21061 tggtcagagg tactgggtat gacaaagtat gatgaatagt tttagaggag tacagggtag 21121 gtaggaaggg tagagatgta gaatgttagg caggatgtgg aagctatgaa gggctaatcc 21181 agactctcag ggctctattc taaagaggtt ggacaatatc ttatgagtaa cagaggacca 21241 cagtgatagc taagcagagg tgtgctatta ataattagct ttgaggaaga taatctatga 21301 gatacggagc atgggcaatg gggcagggaa aagggtagag ggggcaaagt cagcaagagg 21361 tgacaaaaag accattcccc cagtagggaa gctgtttatc ttattagctt tgaatagaaa 21421 tctgaaaatt acatgcatac tttggcctgg gctctgggag aagacaatgg ctttagagca 21481 ataaaatgtg ctttcggttc ttcttctctt tttttttgaa ttggaatctg ctatgttgcc 21541 cagctggtct caaaatcctg ggctcaaaag atcctcctac cttggcctcc tgagtagctg 21601 ggactagagc catgaaccac tacacctagc ccttttagtt ctaactagat tgctttctac 21661 ttgacatttt tctttccatt gtactgcaac atctatgaac tttgtctgat tttggtgtaa 21721 ttctaaaagg tattaattta aagatcacaa taaaataaaa tcacgttcaa tataaaagca 21781 ttaagctgga aaatgcttta tttttttaag ggaaaagcac aatgaaggta aaactgttag 21841 ggaaatttgt gagtcaaata atattttgtt gaaatatata aagaaaaaat gattggaggc 21901 agaaggataa attgacgtaa gtacaattgt aaattgggga tttttaaaaa tttattttat 21961 ttatttattt atttttttga gacagagtct cgctgtgtcg tcaggctgga gtgcagtggt 22021 acaatctcgg ctcactgcaa gctctgactc cctggttcaa gcgattctcc tacctcagcc 22081 tcctgagtag ctgggattac aggcacgcgc catgacaccc agctaatttt tgtattttta 22141 gtagagacgg ggtttcacca tgttgg //