HandsOn 18 - Bacterial Growth

I. Bacterial Growth Preparation

II. Streaking and Inoculating Plates

III. Growth of Bacteria Under Starvation Conditions

IV. Staining Bacterial Colonies

IV. Staining Bacterial Colonies

If you want to preserve your bacterial colony, you can stain it. Staining the colony kills the bacteria to prevent further growth, and makes the colony easier to look at both with the naked eye and under a microscope.

Note of Caution: Please note that the methanol and acetic acid used in the staining solution are hazardous. To prevent damage to the mucus membrane, a fume hood should be used. Gloves should be worn to prevent skin irritation and neither chemical should be ingested. Be sure to keep the Material Safety Data Sheets provided by the chemical supply company. You will also be working with a strong staining agent. Be sure to take precautions regarding skin and clothing.

You will need:

To prepare 1 liter of staining solution (be sure to follow the exact order of these steps):

The ingredients can be scaled proportionally for smaller amounts of staining solution. Use the base proportions of (4:5:1) distilled water:methanol:acetic acid.

The rinse solution can be made by following Steps 1-3 for making the staining solution. Be sure not to add the stain powder to your rinse solution.

Note of Caution: When disposing of unwanted bacterial colony plates, you must first autoclave them. This will kill the bacteria, and make the plates safe for disposal in a regular trash bag.

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